CYTOKINE REGULATION OF PROLIFERATIVE ACTIVITY OF PERIPHERAL BLOOD CELLS

Abstract

The purpose of the research was to study effects of various cytokines on proliferation of peripheral blood white cells. There has been carried out an immunological examination of 234 healthy people aged 20 to 50 years, which included a study of immunograms, hemograms, neutrograms, monocytograms and lymphocytograms. In serum with use of ELISA method, there was determined content of tumor markers: carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP), as well as cytokines: IL-6, IL-4, IL-10, TNF-α and IFN-γ. The same type of inhibitory effect of elevated concentrations of cytokines, the mechanism of which is probably the induction of expression of tyrosine kinases has been shown. It has been shown that cytokines in concentrations that did not exceed the physiological norms and were close to the average content increased proliferation and differentiation of T and B-lymphocytes thus stimulating cell-mediated and antibody-dependent immune responses. High concentrations of proinflammatory cytokines lead to a sharp reduction in the number of peripheral blood cells with markers of both early and late activation of proliferation without further development of cell-mediated and antibody-dependent responses. Increased concentrations of IL-6 and TNF-α in blood serum more than 20 pg / ml reduced content of CEA and AFD, respectively.