Diurnal dynamics of rat blood electrolytes at constant lighting and chronic alcohol intoxication

Abstract

BACKGROUND: Violation of the lighting regime, as well as alcohol abuse, is significant factors in the disorganization of biorhythms in the modern world. Violation of rhythm leads to a mismatch of body functions, as a result to disadaptation, the occurrence of desynchronosis and the development of a number of socially significant diseases. AIM: Study of influence of constant lighting, chronic alcohol intoxication and joint action of these factors on diurnal dynamics of several electrolytes in blood of rats. MATERIALS AND METHODS: The study was conducted on 160 male of Wistar outbred stock at age of 6 months, with body weight of 300 20 g, which were divided into 4 groups. Control group animals, which were kept under conditions of fixed light regime (light:darkness = 10:14 hours), I group animals, which were kept under conditions of fixed light regime, but received a 15% water solution of ethanol as a drink, II group animals, which were kept under conditions of constant light regime, III group animals, which were kept under conditions of constant light regime and received a 15% water solution of ethanol as a drink. In blood samples, taken at 9:00, 15:00, 21:00 and 3:00 oclock, the concentration of total calcium, potassium, sodium, chlorine and phosphorus was measured. The reliability of circadian rhythmicity was determined through cosinor analysis. RESULTS: The increase in the concentration of phosphorus in the blood plasma of animals of II and III experimental groups was found with the decrease in the concentration of total calcium in animals of I and III experimental groups. Constant lighting and chronic alcohol intoxication affect the amplitude-phase characteristics of the circadian rhythms of all studied ions, and the combined action of these factors causes the destruction of the circadian rhythms of the studied electrolytes. CONCLUSIONS: It is established that the three-weeks-long influence of the studied chronodestructors leads to disruptions in metabolism of calcium and phosphorus, which are the most pronounced at joint action of darkness deprivation and chronic alcohol intoxication, and also to the significant disruptions in the structure of CRs of all of studied substances.