Author:
Ofitserova Ekaterina S.,Alekseeva Lilia N.,Shklyarenko Artem A.,Yakovlev Igor P.
Abstract
Topicality. This article presents the results of the study of the biological activity of newly synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice with a total weight of 1620 g. A generation model was used to experimentally evaluate the analgesic activity acetic writhing in male mice. It was experimentally confirmed that the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were non-toxic and had pronounced analgesic activity.
Aim: to study the biological activity of the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines.
Materials and methods. The prediction of the likely types of biological activity was carried out with the use of PASS computer program. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice. Analgesic activity was established on a model of acetic acid writhing in mice.
Results. 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were classified as non-toxic substances (LD50 2000 mg / kg). The analgesic activity of the test compounds was higher than that of the reference drug.
Conclusion. The new synthesized 6-[(1-naphthylmethyl]thio)-4-chloropyrazolo[3,4-d]pyrimidines are non-toxic and have pronounced analgesic activity.
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