Author:
Nesterova Irina V.,Khalturina E. O.,Nelyubin V. N.,Khaidukov S. V.,Chudilova Galina A.,Malinovskaya V. V.
Abstract
Introduction. Neutrophil granulocytes (NGs) and the interferons system (IFN) play a leading role in antiviral immune defense. At the same time, patients suffering from atypical chronic active herpes-viral infections (ACA-HVI) often have neutropenia and impaired induced IFN/ and IFN production. It is possible that these patients have various disorders of intracellular signaling at all stages of IFN/ synthesis by immune system cells, including NG, which leads to IFN/ deficiency. An adequate level of nuclear factor NF-kB expression has positive effects on synthesis of IFN/ in the last stages of signaling. The disturbances in the expression of NF-kB can lead to defects in the synthesis of IFN/.
The aim: to clarify the features of nuclear factor NF-kB, membrane receptors IFN|R and IFNR (CD119) expression on NG of patients with AСA-HVI, with the subsequent assessment of the recombinant IFN2b (recIFN2b) effects on them in the experimental system in vitro.
Materials and methods: 25 patients with ACA-HVI of both sexes aged 23-64 years were included in the main group of the study (MSG). In the complex of the study for the detection of herpes-viral infections: the method of serodiagnosis (ELISA), for the detection of the genome of viruses - PCR-RT. 407 blood samples were examined in the in vitro system. Flow cytofluorimetry was used to estimate the amount (%) of NG expressing NF-kB, IFN|R, IFNR, and their expression levels before and after incubation with recIFN2b. Adequate statistical methods were applied.
Results: In patients suffering from ACA-HVI, a deficiency of induced IFN and IFN production was detected, due to a decrease in the expression density of nuclear factor NF-kB NG, as well as a violation of the expression of membrane receptors IFN|R, IFNR. RecIFN2b in the in vitro system had a mixed effect on the expression of NF-kB, IFN|R and IFNR NG.
Conclusion: The deficiency of induced IFN and IFN production in patients with ACA-HVI is associated with variable changes in NF-kB, IFN|R and IFNR NG expression. RecIFN2b in the in vitro system has an ambiguous effect on altered expression of NF-kB, IFN/ and IFN NG, which appears to depend on the innate or adaptive nature of these disorders.
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