Author:
Kobyakov Dmitriy S.,Avdalyan A. M,Lazarev A. F,Lushnikova E. L,Nepomnyaschikh L. M
Abstract
Purpose. Study of the content ofprotein HER2 and gene HER2 , CEP17 in conjunction with clinical and morphological parameters and survival in non-small cell lung cancer. Material and methods. Investigated 218 surgery samples of non-small cell lung cancer. HER2 protein was determined by immunohistochemistry (clone 4B5, «Ventana») andgene HER2, CEP17 by hybridization in situ (SISH, «Ventana»). Results. Found relationship between the clinical and morphological parameters on the TNM system with protein HER2 status and CEP17 (for value N), protein HER2 status and gene HER2 amplification (for tumor histogenesis). Survival ofpatients with protein HER2 negative status better than positive status. Found better survival in the absence of gene HER2 amplification and increase CEP17 or gene HER2 amplification in conjunction with increase CEP17, the worse survival - gene HER2 amplification or increase CEP17. In univariate regression analysis protein HER2 status had an impact on survival. Multivariate regression analysis showed no relationship protein HER2 status and gene HER2, CEP17 with patient survival. Conclusion. The content ofprotein HER2 and gene HER2, CEP17 correlated with clinical and morphological parameters and survival of non-small cell lung cancer patients.
Reference22 articles.
1. Reinmuth N., Brandt B., Kunze W.P., Junker K., Thomas M., Achatzy R. et al. Ploidy, expression of erbB1, erbB2, p53 and amplification of erbB1, erbB2 and erbB3 in non-small cell lung cancer. Eur. Respir. J. 2000; 16: 991-6.
2. Hofmann M., Stoss O., Shi D., Buttner R., van de Vijver M., Kim W. et al. Assessment of a HER2 scoring system for gastric cancer: results from a validation study. Histopathology. 2008; 52: 797-805.
3. Schraml P., Kononen J., Bubendorf L., Moch H., Bissig H., Nocito A. et al. Tissue microarrays for gene amplification surveys in many different tumor types. Clin. Cancer. Res. 1999; 5: 1966-75.
4. Au N.H., Cheang M., Huntsman D.G., Yorida E., Coldman A., Elliott W.M. et al. Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers. J. Pathol. 2004; 204: 101-9.
5. Cappuzzo F., Varella-Garcia M., Shigematsu H., Domenichini I. , Bartolini S., Ceresoli G.L. et al. Increased HER2 gene copy number is associated with response to gefitinib therapy in epidermal growth factor receptor-positive non-small-cell lung cancer patients. J. Clin. Oncol. 2005; 23: 5007-18.