Methods of functional neurohistology and their practical application

Abstract

Functional neurohistology is a field of morphological research that uses microscopic methods to assess the morphological support of the functions of brain structures, nerve tissue and nerve cells. These include classical histological methods, electron microscopy, histochemistry and immunohistochemistry in combination with morphometry. Each of the methodological approaches has its advantages and disadvantages. The choice of methods of functional neurohistology is determined by the specific objectives of the study. The review describes the methodological approaches used by us to assess the functional state of brain neurons in normal and pathological conditions. The advantages and necessity of using a complex of microscopic methods for a more complete assessment of the state of neurons are substantiated.