Author:
Shirshin E.A.,Yakimov B.P.,Budylin G.S.,Buyankin K.E.,Armaganov A.G.,Fadeev V.V.,Kamalov A.A.
Abstract
AbstractOptical methods are widely used to perform fundamental studies of living systems and solve problems of biomedical diagnostics. Along with the classical spectroscopy, methods of nonlinear optics (e.g., multiphoton microscopy) are also applied in biophotonics. The potential of nonlinear optical methods for visualisation and analysis of the properties of endogenous chromophore molecules are considered in this minireview. Melanin – a pigment with specific spectral features of photophysical properties in the visible and near-IR ranges – is taken as an example. It is discussed what information about its localisation in tissues and structural organisation can be obtained by nonlinear optical methods: multiphoton fluorescence microscopy (including fluorescence lifetime imaging), third harmonic generation, pump – probe spectroscopy, and coherent anti-Stokes Raman spectroscopy.
Subject
Electrical and Electronic Engineering,Atomic and Molecular Physics, and Optics,Statistical and Nonlinear Physics,Electronic, Optical and Magnetic Materials
Cited by
1 articles.
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