STUDYING THE COMPOSITION OF THE LIPID COMPLEX ARCTIUM LAPPA L. FRUITS BY METHODS OF CHROMATO-MASS SPECTROMETRY AND 1H NMR SPECTROSCOPY-Reference-Cited by-同舟云学术

STUDYING THE COMPOSITION OF THE LIPID COMPLEX ARCTIUM LAPPA L. FRUITS BY METHODS OF CHROMATO-MASS SPECTROMETRY AND 1H NMR SPECTROSCOPY

Published:2024-08-06 Issue: Volume: Page:22-29
ISSN:2587-7313
Container-title:Problems of Biological, Medical and Pharmaceutical Chemistry
language:
Short-container-title:Problems Bio Med Pharm Chem

Author:

Babaeva E.Yu.¹^ORCID,Goriainov S.V.²^ORCID,Burova A.E.³^ORCID,Vandishev V.V.⁴^ORCID,Ivlev V.A.⁵^ORCID,Kalabin G.A.

Affiliation:

1. Ph.D. (Biol.), Associate Professor, Leading Research Scientist E-mail: babaevaelena@mail.ru; ORCID 0000-0002-4992-6926 All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), Grina str. 7, Moscow, 117216, Russian Federation

2. Head of the Department of Gas Chromatography, Mass Spectrometry and NMR Spectroscopy, RERC «Pharmacy» E-mail: goryainovs@list.ru; ORCID 0000-0002-7625-9110 Peoples Friendship University of Russia named after Patrice Lumumba, Mikluho-Maklaya str., 8, Moscow, 117198, Russian Federation

3. Senior Research Scientist Peoples Friendship University of Russia named after Patrice Lumumba, Mikluho-Maklaya str., 8, Moscow, 117198, Russian Federation

4. Educational Master, Department of General Pharmaceutical and Biomedical Technology Peoples Friendship University of Russia named after Patrice Lumumba, Mikluho-Maklaya str., 8, Moscow, 117198, Russian Federation

5. Chief Specialist, Department of Gas Chromatography, Mass Spectrometry and NMR Spectroscopy, RERC «Pharmacy» Peoples Friendship University of Russia named after Patrice Lumumba, Mikluho-Maklaya str., 8, Moscow, 117198, Russian Federation

Abstract

Introduction. The pharmacopoeial medicinal raw material of burdock (Arctium lappa L.) are roots. The plant has recently been introduced into the crop into cultivation and the fruits are the sowing material. If the safety stock is not used, it can be used to isolate the lipid complex. The goal is to study the component composition of the lipid complex of burdock fruits. Material and Methods. Burdock fruits harvest of 2012-2015 from the biocollection of the Federal State Budgetary Institution All-Russian Scientific Re-search Institute of Medicinal and Aromatic Plants (Central region of the Non-Chernozem Zone of the Russian Federation) were studied. The lipid complex was isolated with hexane and analyzed by gas chromatography-mass spectrometry and 1H NMR spectroscopy. Both methods are pharmacopoeial. Results. It was established that the content of the lipid complex in the studied fruits was no less than 24.0%. The profiles and contents of fatty acids, tocopherols, sterols, triterpene alcohols, and lignans have been established. The saponified portion of lipids by triglycerides (98.9%) is represented, di- (0.7%) and monoglycerides (0.4%) in minor quantities are present. They include predominantly linoleic (59.9%), oleic (21.9%), palmitic (8.3%) and stearic (4.8%) acids. 3.5% of fatty acids by α-linolenic acid and other unsaturated acids with three double bonds are represented. Among the lignan fraction of the lipid complex, arctiin dominates (2.2%), the content of tocopherols, up to 70% of which δ-tocopherol (0.07%) is represented. Sterols and triterpene alcohols make up 0.7%, of which up to 64% consist of β-sitosterol (28.8%), Δ7-sitosterol (13.2%), cycloartenol (11.6%) and citrostadienol (10.8 %). Conclusions. The fruits of burdock, cultivated in the Central region of the Non-Chernozem zone of the Russian Federation, are promising medicinal raw materials with a rich composition of biologically active substances.

Publisher

Russian Vrach, Publishing House Ltd.

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