SELECTION OF METHODOLOGY FOR THIOPHAN M ISOLATION FROM ANIMAL ORGANS

Abstract

Introduction. An important preparatory stage of collecting information for the registration of a new drug is the study of its pharmacokinetic parame-ters in accordance with the requirements of good laboratory practice. The preparatory stage of such a study consists in the selection of optimal condi-tions for sample preparation of biological objects to determine the test substance in them. The purpose of this study is to select optimal conditions for sample preparation, including the pH value of the extractant, and isolation of Thio-phane M from the organs of laboratory animals (rats) for its further voltammetric determination according to the previously developed method. Material and methods. The object of the study was the substance Thiophane M [dodecyl(3,5,-dimethyl-4-hydroxybenzyl)sulfide], which is a promis-ing biologically active compound with antioxidant properties, developed on the basis of the Department of Chemistry of Novosibirsk State Pedagogical University together with the Research Institute of Antioxidant Chemistry. Experimental data were obtained using 5 male rats weighing from 350-380 g, which were injected with an intragastrically tested sample of Thiophane M at a dose of 500 mg/kg in the form of an oil solution in olive oil. Results. The total number of samples obtained and examined is 180. Based on the data obtained with their subsequent statistical processing by the indirect differences method according to the State Pharmacopoeia of the XIV edition, it was established that the optimal condition for the extraction of Thiophane M is a single extraction with diethyl ether, which is confirmed by the results of voltammetric analysis. We have found that the greatest number Thiophane M localized in the brain, heart and lungs (364.08.20, 332.016.47 and 275.025.40 µcg/g, respectively), fewer Thiophane M detect-ed in kidney, spleen and liver (146.015.50, 81.08.66 56.014.53 µcg/g, respectively). Conclusions. Of all the methods of Thiophan M isolation from rat organs, the method using diethyl ether as an extractant was chosen, which gave stable results due to the maximum extraction of the substance.