ASSESSMENT OF THE RELATIONSHIP BETWEEN THE CONCENTRATION OF PSGL-1, NO-SYNTHASES AND NITRIC OXIDE METABOLITES IN THE BLOOD SERUM OF PATIENTS WITH ACUTE ARTERIAL THROMBOSIS-Reference-Cited by-同舟云学术

ASSESSMENT OF THE RELATIONSHIP BETWEEN THE CONCENTRATION OF PSGL-1, NO-SYNTHASES AND NITRIC OXIDE METABOLITES IN THE BLOOD SERUM OF PATIENTS WITH ACUTE ARTERIAL THROMBOSIS

Published:2024-09-01 Issue: Volume: Page:16-21
ISSN:2587-7313
Container-title:Problems of Biological, Medical and Pharmaceutical Chemistry
language:
Short-container-title:Problems Bio Med Pharm Chem

Author:

Korotkova N.V.¹,Kalinin R.E.²,Suchkov I.A.³,Mzhavanadze N.D.⁴,Nikiforov A.A.⁵,Fomina S.I.⁶,Alekseenko A.D.⁷

Affiliation:

1. Ph.D. (Med.), Associate Professor of the Department of Biological Chemistry, Senior Research Scientist, Central Research Laboratory Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

2. Dr.Sc. (Med.), Professor, Head of the Department of Cardiovascular, Endovascular Surgery, and Diagnostic Radiology Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

3. Dr.Sc. (Med.), Professor, Professor of the Department of Cardiovascular, Endovascular Surgery, And Diagnostic Radiology Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

4. Dr.Sc. (Med.), Associate Professor, Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology, Senior Research Scientist, Central Research Laboratory Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

5. Ph.D. (Med.), Associate Professor of the Department of Pharmacology, Head of the Central Research Laboratory Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

6. Student, Faculty of Medicine Ryazan State Medical University named after academician I.P. Pavlov, Vysokovoltnaya str., 9, 390026, Ryazan, Russian Federation

7. Student, N.V. Sklifosovsky Institute of Clinical Medicine First Moscow State Medical University (Sechenov University), Trubetskaya str., 8, building 2, 119048, Moscow, Russian Federation

Abstract

Introduction. PSGL-1 is a complex protein, that provides contacts between blood cells and endothelial cells by connecting with cell-adhesion mole-cules selectins, and it is a universal participant in the processes of leukocyte adhesion, inflammation and immune response. The study of the possible regulation of its functioning is an urgent topic, since the process of its interactions with selectins can be a point of therapeutic application. Aim of the study. Evaluation of the relationship between the concentration of universal ligand selectins PSGL-1, inducible (iNOS) and endothelial (eNOS) NO synthases and final metabolites of nitric oxide (II) in the blood serum of patients with acute arterial thrombosis. Material and methods. The concentrations of PSGL-1, iNOS and eNOS in blood serum were determined, using enzyme immunoassay on an enzyme immunoassay analyzer Stat Fax 2100. The total concentration of the final stable metabolites of nitric oxide(II) (nitrates and nitrites) was determined by the spectrophotometric method modified by V.A. Metelskaya, by coloring in the reaction of diazotization with nitrite of sulfonamide present in the com-position of the Griss reagent. The results were processed, using nonparametric statistics methods of Microsoft Office Excel 2016 and IBM SPSS Statistics 26 (StatSoftInc., USA). Results. The study revealed a statistically significant increase in the level of endothelial nitric oxide synthase and the final stable metabolites of nitric oxide in the blood of patients with acute arterial thrombosis, compared with the control group. A direct correlation of moderate severity was also found between the level of eNOS and the concentration of nitrates and nitrites. A low positive correlation was found between the concentration of PSGL-1 and the level of eNOS, and a low negative correlation was found between PSGL-1 and iNOS. Conclusions. According to the results of the study, it can be concluded that nitric oxide, which generated by endothelial NO synthase in patients with arterial thrombosis, may have a regulatory effect on the expression or functioning of the universal glycoprotein ligand of selectins – PSGL-1, which re-quires more in-depth study.

Publisher

Russian Vrach, Publishing House Ltd.

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