The composition and content of some vitamins in the leaves of European dewberry (Rubus caesius L.) and aqueous extracts based on them

Author:

Ilina M.B.1,Sergunova E.V.1

Affiliation:

1. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya str., 8/2, Mosсow, 119991, Russian Federation

Abstract

Introduction. Vitamins play an important role for the normal functioning of the human body. Some of them are synthesized by the microflora in the intestine, but most of them must enter the body from the outside. In addition to food, medicinal plant raw materials are also a source of vitamins. An example of such raw materials are the leaves of European dewberry, which have been little studied at the moment and are of interest for research. Objective. The study of the composition and content of vitamins in aqueous extraction from the leaves of European dewberry (Rubus caesius L.). Material and methods. The object of the study was an aqueous extraction from the leaves of European dewberry (infusion), prepared according to the rules for the preparation of infusions and decoctions specified in the GPM.1.4.1.0018 “Infusions and decoctions” SP XV. The detection and quantification of vitamins in the sample was carried out by the HPLC method, according to the GPM.1.2.3.0017.15 SP XIV on the Waters 1525 chromatograph. Results and discussion. During the study, 7 compounds were identified in the infusion of European dewberry leaves: vitamin C, vitamin P (rutin), vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (nicotinamide), vitamin B6 (pyridoxine), vitamin B9 (folic acid). The predominant compound in the studied object was ascorbic acid (vitamin C), the content of which is 67.79 mg%. The vitamin content was recalculated for dry raw materials. It has been shown that the amount of water-soluble vitamins and rutin in the leaves of European dewberry is 11.4 times higher than in the infusion. Conclusion. The composition and content of vitamins in the aqueous extraction from the leaves of the European dewberry by HPLC were determined. The presence of 5 B vitamins, ascorbic acid and rutin has been proven. This is the first time such research has been conducted. The leaves of the European dewberry are rich in water-soluble vitamins and rutin, and this raw material can be considered as a vitamin remedy.

Publisher

Russian Vrach, Publishing House Ltd.

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