CARBONYLATION OF SPERMОPLASM PROTEINS IN PATIENTS WITH REDUCED FERTILITY-Reference-Cited by-同舟云学术

CARBONYLATION OF SPERMОPLASM PROTEINS IN PATIENTS WITH REDUCED FERTILITY

Published:2023-02-11 Issue:1 Volume:21 Page:60-64
ISSN:2499-9490
Container-title:Molekulyarnaya Meditsina (Molecular medicine)
language:
Short-container-title:Mol Med Rus

Author:

Ishtulin A.F.¹,Korotkova N.V.¹,Matveeva I.V.¹,Ishtulina S.L.¹,Minaev I.V.¹,Ishchenko Е.А.¹

Affiliation:

1. Ryazan State Medical University, Vysokovoltnaya, 9, Ryazan, 390026, Russian Federation

Abstract

Introduction. In the publications of many authors, there is evidence that the mechanism of development of chronic prostatitis and varicocele is oxidative stress, leading to a decrease in male fertility. This problem requires a deeper study. The aim of the study. To evaluate the carbonylation of spermоplasm’s proteins in patients with chronic prostatitis III B and varicocele II and III degrees with concomitant asthenozoospermia in the anamnesis. Methods. Determination of the total protein concentration in the spermoplasm was carried out by the biuretic method using commercial kits of the company (Mindrey, China) on a biochemical analyzer (Mindrey BS 120, China). Carbonylated proteins in the spermoplasm were evaluated using the R.L. Levine method modified by E.E. Dubinina. Results. In the course of our study, it was found that in patients with chronic prostatitis III B, accompanied by asthenozoospermia and in patients with varicocele II and III degrees with concomitant asthenozoospermia, an increase in carbonylated proteins in the spermoplasm is noted at all absorption maximum (λ356, λ370, λ430, λ30), on three of them statistically significant; at the same time, the reserve-adaptive potential in relation to the oxidative effect is reduced. Thus, the established decrease in fertility in patients with the studied pathology is associated with an increase in carbonylated proteins in the spermoplasm

Publisher

Russian Vrach, Publishing House Ltd.

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