Determination of Serum Leptin Levels in Cattle, Sheep, Goats and Buffaloes in Burdur Province in Türkiye by ELISA Method

Abstract

Leptin is a hormone that provides the central and/or peripheral regulation of food intake, energy expenditure, whole body energy balance, fertility and immune functions by creating a feeling of satiety in animals and humans. This study was carried out to determine serum leptin values for some ruminant species and breeds that were healthy and had at least one birth. In the study, 15 Simental and 15 Holstein cows, 15 Italian buffalo, 15 Pırlak sheep, 15 Honamlı and 15 Turkish hair goats were used as material. Serum leptin concentrations were measured with the BT LAB Bovine Leptin ELISA kit. Serum leptin levels were determined as 2.78±0.22 ng/ml in Simmental cows, 2.87±0.11 ng/ml in Holstein cows, 3.6±0.48 ng/ml in Pırlak sheep, 3.38±0.76 ng/ml in Honamlı goats, 5.48±0.92 ng/ml in Hair goats and 2.50±0.17 ng/ml in Italian buffaloes. As a result, it was determined that serum leptin levels obtained from different ruminant species that were healthy and gave at least one birth in Burdur province were within the reference values reported for ruminants.