Short-term effectiveness of transcranial direct current stimulation in the nociceptive behavior of neuropathic pain rats in development-Reference-Cited by-同舟云学术

Short-term effectiveness of transcranial direct current stimulation in the nociceptive behavior of neuropathic pain rats in development

Published:2023 Issue:4 Volume:10 Page:433-446
ISSN:2373-7972
Container-title:AIMS Neuroscience
language:
Short-container-title:AIMSN

Author:

Crespo Priscila Centeno¹²,Anderson Meira Martins Leo³,Martins Otávio Garcia²,Camacho Dos Reis Clara²,Goulart Ricardo Netto²,de Souza Andressa⁴,Medeiros Liciane Fernandes⁵⁴,Scarabelot Vanessa Leal⁵⁶,Gamaro Giovana Duzzo¹,Silva Sabrina Pereira¹,de Oliveira Marcos Roberto⁷,Torres Iraci Lucena da Silva³⁵,de Souza Izabel Cristina Custódio¹²

Affiliation:

1. Postgraduate Program in Biochemistry and Bioprospection, Universidade Federal de Pelotas, Pelotas (UFPel), Pelotas, Rio Grande do Sul (RS), Brazil

2. Laboratory of Cellular Neuromodulation: Basic Sciences, Institute of Biology, Department of Morphology, UFPel, Pelotas, RS, Brazil

3. Department of Physiology, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

4. Postgraduate Program in Health and Human Development, Universidade La Salle, Canoas, RS, Brazil

5. Laboratory of Pain Pharmacology and Neuromodulation: Preclinical Researches, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil

6. Postgraduate Program in Medicine Medical Sciences, Medicine School, UFRGS, Porto Alegre, RS, Brazil

7. Department of Biochemistry, UFRGS, Porto Alegre, RS, Brazil

Abstract

<abstract> <p>Neuropathic pain (NP) is caused by a lesion that triggers pain chronification and central sensitization and it can develop in a different manner, dependent of age. Recent studies have demonstrated the efficacy of transcranial direct current stimulation (tDCS) for treating NP. Then, we aimed to investigate the effects of tDCS and BDNF levels in neuropathic pain rats in development, with 30 days old in the beginning of experiments. Eight-five male <italic>Wistar</italic> rats were subjected to chronic constriction injury. After establishment of NP, bimodal tDCS was applied to the rats for eight consecutive days, for 20 minutes each session. Subsequently, nociceptive behavior was assessed at baseline, 14 days after surgery, 1 day and 7 days after the end of tDCS. The rats were sacrificed 8 days after the last session of tDCS. An increase in the nociceptive threshold was observed in rats in development 1 day after the end of tDCS (short-term effect), but this effect was not maintained 7 days after the end of tDCS (long-term effect). Furthermore, brain derived neurotrophic factor (BDNF) levels were analyzed in the frontal cortex, spinal cord and serum using ELISA assays. The neuropathic pain model showed an effect of BDNF in the spinal cord of rats in development. There were no effects of BNDF levels of pain or tDCS in the frontal cortex or serum. In conclusion, tDCS is an effective technique to relieve nociceptive behavior at a short-term effect in neuropathic pain rats in development, and BDNF levels were not altered at long-term effect.</p> </abstract>

Publisher

American Institute of Mathematical Sciences (AIMS)

Subject

General Neuroscience

Reference42 articles.