Proteomic and transcriptomic analyses of <i>Cutibacterium acnes</i> biofilms and planktonic cultures in presence of epinephrine

Author:

Gannesen AV1,Schelkunov MI23,Ziganshin RH4,Ovcharova MA1,Sukhacheva MV1,Makarova NE2,Mart'yanov SV1,Loginova NA1,Mosolova AM15,Diuvenji EV1,Nevolina ED1,Plakunov VK1

Affiliation:

1. Federal Research Centre “Fundamentals of Biotechnology” of Russian Academy of Sciences, Moscow 119071, Russia

2. Skolkovo Institute of Science and Technology, Moscow 121205, Russia

3. Institute for Information Transmission Problems of Russian Academy of Sciences, Moscow 127051, Russia

4. Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia

5. Russian Biotechnological University, Moscow 125080, Russia

Abstract

<abstract> <p>Transcriptomic and proteomic analysis were performed on 72 h biofilms of the acneic strain <italic>Cutibacterium acnes</italic> and planktonic cultures in the presence of epinephrine. Epinephrine predominantly downregulated genes associated with various transporter proteins. No correlation was found between proteomic and transcriptomic profiles. In control samples, the expression of 51 proteins differed between planktonic cultures and biofilms. Addition of 5 nM epinephrine reduced this number, and in the presence of 5 µM epinephrine, the difference in proteomic profiles between planktonic cultures and biofilms disappeared. According to the proteomic profiling, epinephrine itself was more effective in the case of <italic>C. acnes</italic> biofilms and potentially affected the tricarboxylic acid cycle (as well as alpha-ketoglutarate decarboxylase Kgd), biotin synthesis, cell division, and transport of different compounds in <italic>C. acnes</italic> cells. These findings are consistent with recent research on <italic>Micrococcus luteus</italic>, suggesting that the effects of epinephrine on actinobacteria may be universal.</p> </abstract>

Publisher

American Institute of Mathematical Sciences (AIMS)

Reference58 articles.

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