Affiliation:
1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
2. Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand
Abstract
<abstract>
<p>The diversity of duckweed (<italic>Lemnaceae</italic>) associated yeasts was studied using a culture-dependent method. A total of 252 yeast strains were isolated from 53 duckweed samples out of the 72 samples collected from 16 provinces in Thailand. Yeast identification was conducted based on the D1/D2 region of the large subunit (LSU) rRNA gene sequence analysis. It revealed that 55.2% and 44.8% yeast species were Ascomycota and Basidiomycota duckweed associated yeasts, respectively. Among all, <italic>Papiliotrema laurentii</italic>, a basidiomycetous yeast, was found as the most prevalent species showing a relative of frequency and frequency of occurrence of 21.8% and 25%, respectively. In this study, high diversity index values were shown, indicated by the Shannon-Wiener index (<italic>H′</italic>), Shannon equitability index (<italic>E<sub>H</sub></italic>) and Simpson diversity index (<italic>1-D</italic>) values of 3.48, 0.86 and 0.96, respectively. The present results revealed that the yeast community on duckweed had increased species diversity, with evenness among species. Principal coordinate analysis (PCoA) revealed no marked differences in yeast communities among duckweed genera. The species accumulation curve showed that the observed species richness was lower than expected. Investigation of the plant growth promoting traits of the isolated yeast on duckweed revealed that 178 yeast strains produced indole-3-acetic acid (IAA) at levels ranging from 0.08–688.93 mg/L. Moreover, siderophore production and phosphate solubilization were also studied. One hundred and seventy-three yeast strains produced siderophores and exhibited siderophores that showed 0.94–2.55 activity units (AU). One hundred six yeast strains showed phosphate solubilization activity, expressed as solubilization efficiency (SE) units, in the range of 0.32–2.13 SE. This work indicates that duckweed associated yeast is a potential microbial resource that can be used for plant growth promotion.</p>
</abstract>
Publisher
American Institute of Mathematical Sciences (AIMS)
Subject
Microbiology (medical),Microbiology
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献