Antibacterial activity of Arthrobacter strains isolated from Great Gobi A Strictly Protected Area, Mongolia-Reference-Cited by-同舟云学术

Antibacterial activity of Arthrobacter strains isolated from Great Gobi A Strictly Protected Area, Mongolia

Published:2024 Issue:1 Volume:10 Page:161-186
ISSN:2471-1888
Container-title:AIMS Microbiology
language:
Short-container-title:AIMSMICRO

Author:

Bernacchi Alberto¹,Semenzato Giulia¹,di Mascolo Manuel¹,Amata Sara²,Bechini Angela³,Berti Fabiola³,Calonico Carmela³,Catania Valentina⁴,Emiliani Giovanni⁵,Esposito Antonia⁶,Greco Claudia⁷,Mocali Stefano⁶,Mucci Nadia⁷,Padula Anna⁷,Piccionello Antonio Palumbo²,Nasanbat Battogtokh⁸,Davaakhuu Gantulga⁸,Bazarragchaa Munkhtsetseg⁹,Riga Francesco¹⁰,Augugliaro Claudio¹¹,Puglia Anna Maria¹²,Zaccaroni Marco¹,Renato Fani¹

Affiliation:

1. Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, Italy

2. Department of Biological, Chemical and Pharmaceutical Sciences and Technologies-STEBICEF, University of Palermo, Viale delle Scienze Ed.17, 90128, Palermo, Italy

3. Department of Health Sciences, University of Florence, viale G.B. Morgagni, 48, 50134 Firenze, Italy

4. Department of Earth and Sea Science (DiSTeM), University of Palermo, Viale delle Scienze Blg. 16, Palermo, 90128, Italy

5. Institute for Sustainable Plant Protection (IPSP)—National Research Council (CNR), Via Madonna del Piano 10, Sesto Fiorentino 50019 Florence, Italy

6. Council for Agricultural and Economics Research (CREA) – Agriculture and Environment, Via di Lanciola 12/A, Cascine del Riccio, 50125, Florence, Italy

7. Unit for Conservation Genetics (BIO-CGE), Institute for Environmental Protection and Research, via Ca' Fornacetta, 9, 40064 Ozzano dell'Emilia Bologna, Italy

8. Institute of Biology, Mongolian Academy of Sciences, Peace Avenue-54B, Bayanzurkh District, Ulaanbaatar-13330, Mongolia

9. Mongolian National University of Medical Sciences, S.Zorig street, Ulaanbaatar-14210, Mongolia

10. Italian Institute for Envioronmental Protection and Research, via Vitalino Brancati, 48, 00144, Roma, Italy

11. Wildlife Initiative, Bayangol, 6th Khoroo, Micro District 10, Ulaanbaatar, 210349, Mongolia

12. Consorzio Italbiotec, Piazza della Trivulziana 4/a 20126 Milano

Abstract

<abstract> Desert soil hosts many microorganisms, whose activities are essential from an ecological viewpoint. Moreover, they are of great anthropic interest. The knowledge of extreme environments microbiomes may be beneficial for agriculture, technology, and human health. In this study, 11 <italic>Arthrobacter</italic> strains from topsoil samples collected from the Great Gobi A Strictly Protected Area in the Gobi Desert, were characterized by a combination of different techniques. The phylogenetic analysis, performed using their 16S rDNA sequences and the most similar <italic>Arthrobacter</italic> sequences found in databases, revealed that most of them were close to <italic>A. crystallopoietes</italic>, while others joined a sister group to the clade formed by <italic>A. humicola, A. pascens</italic>, and <italic>A. oryzae</italic>. The resistance of each strain to different antibiotics, heavy-metals, and NaCl was also tested as well as the inhibitory potential against human pathogens (i.e., <italic>Burkholderia</italic> ssp., <italic>Klebsiella pneumoniae, Pseudomonas aeruginosa</italic>, and <italic>Staphylococcus</italic> ssp.) via cross-streaking, to check the production of metabolites with antimicrobial activity. Data obtained revealed that all strains were resistant to heavy metals and were able to strongly interfere with the growth of many of the human pathogens tested. The volatile organic compounds (VOCs) profile of the 11 <italic>Arthrobacter</italic> strains was also analyzed. A total of 16 different metabolites were found, some of which were already known for having an inhibitory action against different Gram-positive and Gram-negative bacteria. Isolate MS-3A13, producing the highest quantity of VOCs, is the most efficient against <italic>Burkholderia cepacia</italic> complex (Bcc), <italic>K. pneumoniae</italic>, and coagulase-negative Staphylococci (CoNS) strains. This work highlights the importance of understanding microbial populations' phenotypical characteristics and dynamics in extreme environments to uncover the antimicrobial potential of new species and strains. </abstract>

Publisher

American Institute of Mathematical Sciences (AIMS)

Reference42 articles.