Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae

Author:

Karamzadeh Tahereh1,Alipour Hamzeh12,Shahriari-Namadi Marziae2,Raz Abbasali3,Azizi Kourosh1,Bagheri Masoumeh2,Moemenbellah-Fard Mohammad D.12

Affiliation:

1. Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran

2. Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran

3. Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

Abstract

AbstractLarval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.

Publisher

Georg Thieme Verlag KG

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