Abstract
The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably <i>Botryosphaeria dothidea</i>, and <i>Diaporthe</i> species. In this study, a recombinase polymerase amplification (RPA) assay was developed for the rapid and sensitive detection of the pathogens responsible for posing significant threats to the kiwifruit industries. The RPA primer pairs tested in this study were highly specific for detection of <i>B. dothidea</i> and <i>D. eres</i>. The detection limits of our RPA assays were approximately two picograms of fungal genomic DNA. The optimal conditions for the RPA assays were determined to be at a temperature of 39°C maintained for a minimum duration of 5 min. We were able to detect the pathogens from kiwifruit samples inoculated with a very small number of conidia. The RPA assays enabled specific, sensitive, and rapid detection of <i>B. dothidea</i> and <i>D. eres</i>, the primary pathogens responsible for kiwifruit rots in South Korea.
Funder
Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries
Ministry of Agriculture, Food and Rural Affairs
Publisher
Korean Society of Plant Pathology
Subject
Agronomy and Crop Science
Cited by
1 articles.
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