Effective Control Strategy against Bacterial Blight on Carrot

Abstract

Bacterial blight of carrot caused by <i>Xanthomonas hortorum</i> pv. <i>carotae</i> (<i>Xhc</i>) is one of the serious diseases of carrot, of which control measures has not been still established in the domestic farm. In this study, in order to select effective sterilizer for bacterial blight of carrots, three antibiotics such as streptomycin, oxolinic acid, kasugamycin, two copper compounds like copper hydroxide and copper sulfate basic and three rhizobacteria <i>Burkholderia gladioli</i> MRL408-3, <i>Pseudomonas fluorescens</i> TRH415-2 and <i>Bacillus cereus</i> KRY505-3 were selected to investigate their direct antibacterial effects using artificial media, aiming to identify effective pesticides against <i>Xhc</i>. Among them, treated medium with antibiotics such as streptomycin, oxolinic acid, and the antagonistic rhizobacteria MRL408-3 were formed inhibition zone. The agrochemicals and the rhizobacteria MRL408-3, which showed antibacterial effects on carrot leaves, pre-treated on the carrot leaves and then inoculated with <i>Xhc</i>. High control effects were shown on the carrot leaves pre-treated with both streptomycin and oxolinic acid. Scanning electron microscopy images of the carrot leaf surfaces showed that the population of bacteria decreased significantly on leaves pre-treated with streptomycin and oxolinic acid. From these results, it can be inferred that antibiotics like streptomycin and oxolinic acid exhibit superior control effects compared to other agents. This study provides valuable insights towards establishing an effective control system for bacterial blight of carrot.