Affiliation:
1. OSMANIYE KORKUT ATA UNIVERSITY, KADİRLİ ACADEMY OF APPLIED SCIENCES, DEPARTMENT OF GASTRONOMY AND CULINARY ARTS, GASTRONOMY AND CULINARY ARTS PR.
2. CUKUROVA UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF FOOD ENGINEERING, DEPARTMENT OF FOOD TECHNOLOGY PR.
3. CUKUROVA UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL MICROBIOLOGY, MEDICAL MICROBIOLOGY (MEDICINE)
Abstract
The assessment of antibiotic resistance and related genes of foodborne Acinetobacter spp. and the analysis of whether they are genetically related to clinical infection-agent strains are crucial in terms of sustainability of food safety. The study at hand investigated antibiotic resistance, aminoglycoside-modifying enzyme (AME), and colistin resistance (PmrA) genes, clonal relationships while evaluating a possible correlation between antibiotic resistance and related genes between 27 foodborne and 50 clinical Acinetobacter spp. in Turkey. Antimicrobial susceptibilities, (AME), PmrA genes, and clonal relatedness of the strains were performed by disc diffusion, PCR, and Pulsed Field gel Electrophoresis (PFGE) methods, respectively. The aph-AI, aph-6, anth (3’’)-I, aadA1, aadB and PmrA genes were found as (24, 48%), (11, 22%), (7, 14%), (1, 2%), (2, 4%), and (46, 92%) respectively in clinical strains. This rate was found as (14, 51.9%), (16, 59.3%), (19, 70.4%), (2, 7.4%), (0, 0%), and (27, 100%) respectively in foodborne isolates. A positive correlation existed between the number of aph-AI gene positivity and trimethoprim-sulfamethoxazole and gentamycin resistance; anth (3’’)-I gene positivity, and colistin resistance; PmrA gene positivity and piperacillin-tazobactam, ceftazidime, meropenem, amikacin, and imipenem resistance in clinical strains (P
Funder
Osmaniye Korkut Ata Üniversitesi
Publisher
Veterinary Journal of Ankara University
Subject
General Veterinary,Animal Science and Zoology