The Phylogenetic Characterization of Cockroaches (Insecta: Blattaria) in Türkiye and Determination of Their Vector Potential for Medically Important Parasites

Abstract

This study was performed to investigate the phylogenetic characters of the cockroaches in XXX region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions, and to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The amplicons obtained were purified and sequenced with PCR primers. According to PCR-RFLP, cockroach species were identified as Blattella germanica (n =105), Blatta orientalis (n = 86), and Periplaneta americana (n = 29). A total of 13 haplotypes were detected and Maximum likelihood (ML) analyses revealed that the sequences of all three species for the three gene regions showed monophyletic structuring. The cockroaches were examined for the presence of parasites. It was determined that of the 58 parasitic forms identified belonged to helminth species 46 (79.3%) and protozoan species and 12 (20.7%). The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The study findings not only contribute to the molecular epidemiology of cockroaches but also confirm that they play an important role as mechanical vectors of protozoan and helminth parasites.