Fast and Simple microRNA Northern Blots-Reference-Cited by-同舟云学术

Fast and Simple microRNA Northern Blots

Published:2009-01 Issue: Volume:2 Page:BCI.S2257
ISSN:1178-6264
Container-title:Biochemistry Insights
language:en
Short-container-title:Biochem Insights

Author:

Tran Nham¹²

Affiliation:

1. The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital and University of Sydney, Sydney, NSW 2006, Australia.

2. The Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW 2006, Australia.

Abstract

RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.

Publisher

SAGE Publications

Link

http://journals.sagepub.com/doi/pdf/10.4137/BCI.S2257

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