Antioxidant and Pro-collagen Synthesis Activities of the Eriobotrya japonica Leaf Extract Using Enzyme

Abstract

Purpose: We conducted a study that examined the physicochemical properties of Eriobotrya japonica leaf enzyme-assisted extract (EJLE) from Jeju Island.Methods: EJLE was prepared using the pepsin enzyme. The antioxidant and antiaging effects of EJLE were investigated to determine its utility as a functional material.Results: In the analysis of total amino acids, 16 kinds of components were isolated from EJLE. The DPPH and ABTS radical scavenging activities of EJLE were found to be 97.19±0.24% and 96.91±0.02% respectively in 500 μg/mL. Inhibition activities of elastase by EJLE were confirmed. In fibroblasts, EJLE at 500 μg/mL demonstrated a procollagen synthesis activity of approximately 52%.Conclusion: Our results confirmed that EJLE induced antioxidants in cosmetic materials and increased procollagen synthesis on the skin.