Anti-aging Effect of Sargassum patens Extract in UVA-irradiated Human Dermal Fibroblasts

Abstract

Purpose: Ultraviolet (UV) irradiation causes extrinsic aging of human skin. It is believed that UV irradiation caused aging phenomena such as skin erythema, wrinkle formation, and skin elasticity reduction.Methods: The effect of Sargassum patens extract on COL1A1 and MMP1 production in normal human dermal fibroblasts was investigated as a potential cosmetic raw material.Results: In this study, I assessed how Sargassum patens extract affected COL1A1/COL3A1/MMP1/SODs/Catalase mRNA expression and protein production of Collagen, type I and MMP1. Quantitative real-time reverse transcription–polymerase chain reaction demonstrated that the Sargassum patens extract increased the mRNA levels of COL1A1/COL3A1/SOD2/CAT genes. Additionally, when compared to UVA-treated dermal fibroblasts, collagen and type I protein increased in Sargassum patens extract-treated conditions. Furthermore, MMP1 mRNA and secreted MMP1 protein expression were reduced by Sargassum patens extract treatment. According to these findings, Sargassum patens extract increased antiaging and antioxidative effects in dermal fibroblasts.Conclusion: Therefore, I identified the antiaging effects of Sargassum patens extract, and these findings indicated that Sargassum patens could be a pharmaceutical ingredient for skin antiaging. Based on this, I expected to conduct additional research on Sargassum patens extract for cellular mechanisms.