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Glycerol Increases the Yield and Activity of Human Phenylalanine Hydroxylase Mutant Enzymes Produced in a Prokaryotic Expression System

  • Published:2001-06 Issue:2 Volume:73 Page:173-178
  • ISSN:1096-7192
  • Container-title:Molecular Genetics and Metabolism
  • language:en
  • Short-container-title:Molecular Genetics and Metabolism

Author:

Leandro Paula,Lechner Maria Celeste,Tavares de Almeida Isabel,Konecki David

Publisher

Elsevier BV

Subject

Endocrinology,Genetics,Molecular Biology,Biochemistry,Endocrinology, Diabetes and Metabolism

Reference18 articles.

1. The hyperphenylalaninemias;Scriver,1995

2. The V388M mutation results in a kinetic variant form of phenylalanine hydroxylase;Leandro;Mol Genet Metab,2000

3. Solubilization of protein aggregates;Marston;Methods Enzymol,1990

4. Molecular chaperones;Ellis;Annu Rev Biochem,1991

5. Glycerol reverses the misfolding phenotype of the most common cystic fibrosis mutation;Sato;J Biol Chem,1996

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