Author:
Asselborn S.A., ,Zatsepin E.S.,Isakov D.S.,Gerasimov A.M.,Pikhulya D.G.,Miklyaev Yu.V.
Abstract
Illumination scheme for superresolution microscopy is developed. The scheme accomplishes dark filed illumination with a laser light source including spatial coherence suppression. The scheme allows to observe nanoparticles with a size smaller than 50 nm. This is necessary to get higher resolution in the previously proposed method of superresolution microscopy (Near field Optical Random Microscopy – NORM). This method is based on real-time video processing of a nanoparticles Brownian motion those are located near the object surface. The method of vertical coordinate measurement is demonstrated. This method is based on astigmatic nanoparticle imaging. Three-dimensional distributions of suspended nanoparticles are obtained. Vertical resolution better than 200 nm and lateral resolution better than 100 nm are demonstrated.
Publisher
FSAEIHE South Ural State University (National Research University)
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