Challenges of Spectrofluorometry, Part 2: Instrument-Specific Concerns-Reference-Cited by-同舟云学术

Challenges of Spectrofluorometry, Part 2: Instrument-Specific Concerns

Published:2021-11-01 Issue: Volume: Page:41-45
ISSN:1939-1900
Container-title:Spectroscopy
language:en
Short-container-title:Spectroscopy

Author:

English Caitlyn¹,Kitzhaber Zechariah¹,Williams Joshua¹,Myrick M.L.¹

Affiliation:

1. University of South Carolina

Abstract

Part 1 of this three-part series provided a procedure for quickly recording acceptable fluorescence spectra with classic commercial spectrofluorometers for most samples in common 1-cm-pathlength cuvettes. Part 2 of the series describes instrument-specific concerns that require modifications to our initial procedure if we want spectra that can be accurately reproduced in other laboratories. Part 3 will discuss how the sample itself can make reproducibility between instruments challenging.

Publisher

Multimedia Pharma Sciences, LLC

Subject

Spectroscopy,Atomic and Molecular Physics, and Optics,Analytical Chemistry

Reference5 articles.

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2. P.C. DeRose, “Standard Guide to Fluorescence – Instrument Calibration and Validation,” NIST Interagency/Internal Report (NISTIR) – 7458 (National Institute of Standards and Technology, Gaithersburg, MD, 2008, accessed September 27, 2021). https://doi.org/10.6028/NIST.IR.7458

3. J. Paoletti and J-B. Le Pecq, Anal. Biochem. 31, 33–41 (1969). https://doi.org/10.1016/0003-2697(69)90238-3

4. U. Resch-Genger, Eds., Standardization and Quality Assurance in Fluorescence Measurements I (Springer-Verlag, Berlin, 2008), vol. 5.

5. J.A. Gardecki and M. Maroncelli, Applied Spectroscopy 52, 1179–1189 (1998). https://doi.org/10.1366/0003702981945192.

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