Affiliation:
1. Brigham Young University
Abstract
Direct profiling of biochemical expression within single cells provides insights into cellular processes that are lost when ensemble averages are measured across populations of cells. Advanced separations coupled with mass spectrometry (MS) can now quantify more than 1000 proteins within single cells. Further miniaturization of separations will greatly extend the reach of single-cell proteomics, metabolomics, and lipidomics, but key challenges in instrumentation, column technology, and ionization sources must be addressed.
Publisher
Multimedia Pharma Sciences, LLC