Abstract
Ocean fish cells contain trimethylamine oxide for osmoregulation. The odorless trimethylamine oxide reduces to trimethylamine by bacterial activity, resulting in a strong fishy odor. Therefore, it is important to find out whether trimethylamine levels in fish oil and multivitamin softgels containing fish oil will affect the quality of the finished products. A derivatization-based, reversed-phase high performance liquid chromatography (HPLC) method was developed using a Lichosphere 100 RP18 column, utilizing acetonitrile and water as the mobile phases. Experimental products were dissolved in ether, followed by a pH jump and buffer exchange, and finally derivatized with 9-fluorenylmethyl chloroformate. Our research has indicated that the level of trimethylamine in the products containing fish oil does not have a correlation with age and rancidity level of the products.
Publisher
Multimedia Pharma Sciences, LLC
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