Affiliation:
1. Gustavus Adolphus College
Abstract
As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshooting and fixing problems, should also change. In this installment, we continue the discussion started earlier this year about how some specific troubleshooting topics have evolved over time, this time focusing on chemical causes of peak tailing, and strategies that we can use to overcome them. Understanding how knowledge and technologies related to peak tailing have evolved is helpful for troubleshooting problems with peak shape, designing reliability into new methods, and when considering updates to legacy methods that have been in use for decades.
Publisher
Multimedia Pharma Sciences, LLC
Reference14 articles.
1. Dolan, J. W. Basic Compounds - Starting on the Right Foot. LCGC 1999, 17 (2), 100–106.
2. McCalley, D. V.; Stoll, D. R. But My Peaks Are Not Gaussian! Part III: Physicochemical Causes of Peak Tailing. LCGC North Am. 2021, 39 (11), 526-531,539.
3. Dolan, J. W.; Snyder, L. R. Troubleshooting LC Systems: A Comprehensive Approach to Troubleshooting LC Equipment and Separations; Humana Press, 1989.
4. McCalley, D. V. Understanding and Managing Peak Shape for Basic Solutes in Reversed-Phase High Performance Liquid Chromatography. Chem. Commun. 2023, 59 (51), 7887–7899. DOI: 10.1039/D3CC01535A
5. Stoll, D. R. Essentials of LC Troubleshooting, III – Those Peaks Don’t Look Right. LCGC North Am. 2022, 40 (6), 244–247. DOI: 10.56530/lcgc.na.az3781g7