The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS Applications

Abstract

Immobilized enzyme reactors (IMERs) are a powerful and essential part of multidimensional liquid chromatography–tandem mass spectrometry (MDLC–MS/MS) approaches that enable online identification, characterization, and quantification of post-translational modifications of therapeutic antibodies. This review gives an overview of commercially available and selected trypsin IMERs in regard to their application in LC-based and automated sample preparation. Additionally, we address the challenges of IMER application in online systems and the advantages of self-made IMERs.