Resolving Separation Issues with Computational Methods, Part 2: Why is Peak Integration Still an Issue?

Abstract

Our attention is typically focused on the chromatography in the laboratory. Yet ultimately, the liquid chromatograph (LC) or gas chromatograph (GC) only produces a signal—the chromatogram—from which information still must be distilled. In this series, we will discuss different aspects of data analysis and learn how to extract different bits of information from our chromatograms. We start with simple, yet important, bits of information, which we will tie together in future articles. In this installment, we establish why peak integration still poses challenges, and at the same time, see some of the computational techniques in action that we learn to use ourselves in future installments.