Chromosome Mapping of Repetitive DNAs in the Picasso Triggerfish (Rhinecanthus aculeatus (Linnaeus, 1758)) in Family Balistidae by Classical and Molecular Cytogenetic Techniques

Abstract

This work presents the cytogenetic analysis conducted on the Picasso triggerfish (Rhinecanthus aculeatus (Linnaeus, 1758)) from Thailand. Mitotic chromosomes were prepared from the anterior kidney. The cell suspensions were harvested by in vivo colchicine treatment. The present study includes the chromosomal investigation on R. aculeatus, using conventional (Giemsa staining, Ag-NOR and C-banding) and molecular approaches (in situ mapping of five different repetitive DNA classes including 18S rDNA, 5S rDNA, (CA)15, (GA)15 and (CAA)10 as markers.) The results showed that R. aculeatus has karyotypes formed exclusively by telocentric chromosomes (44t; NF=44). The C-positive heterochromatic blocks are preferentially located in the centromeric and telomeric regions of some chromosomal pairs. The Ag-NOR sites occupy the interstitial position of the long arms of the largest telocentric pair (pair 1). The exclusive location of the major ribosomal sites in these pairs was confirmed by hybridization with 18S rDNA probes. However, the 5S rDNA genes are not located on 18S rDNA-bearing chromosomes, but instead located exclusively in the subcentromeric region of pair 4. The mapping of (CA)15, (GA)15 and (CAA)10 microsatellites are sparsely dispersed along all the chromosomes. The karyotype formula of R. aculeatus is 2n (44) = 44t.