Estimation of the polymorphism of matrix metal proteinase genes in patients with endometrioid ovarian cysts

Abstract

Aim. To determine the association of the MMP-9 genetic polymorphism with the risk of developing endometrioid ovarian cysts (ECC). Materials and methods. 55 women aged 19 to 47 were examined. 27 women underwent surgery for ECC (group 1). The control (group 2) consisted of 28 patients without endometriosis and operated because of tubal infertility. Single nucleotide polymorphism was investigated. Genotyping was performed by restriction fragment length polymorphism analysis. Results. We did not find statistically significant differences between the group of patients with ECF and the control group in terms of age 29.0 (25.95; 33.1), 34.5 (29.3; 37.0); p0.05, body mass index 21.2 (19.8; 22.6), 21.95 (20.4; 23.9), p0.05, age of menarche onset 13.0 (12.95; 14.0), 13.0 (12.0; 14.0), p0.05, duration of menstrual bleeding 5.0 (5.0; 5.0), 5.0 (5.0; 6.0), p0.05 for the 1st and 2nd groups, respectively, also dysmenorrhea (2 0.019; p=0.8918), the number of births (2 3.441; p=0.3285) and abortions (2 2.822; p=0.0930) in anamnesis. The frequencies of all studied genotypes of metalloproteinase MMP-9 C (1562) T of the MMP9 gene in the group of patients with ECF and the control group are in HardyWeinberg equilibrium (p=0.99, p=0.43 for 1 and 2 group) which excludes differences in the distribution of genotype frequencies of polymorphic loci of MMP9 genes in the group of patients with ECF and the control group. However, the result could be influenced by the severity of the disease and the size of the study groups. Conclusion. Given the undoubted role of matrix metalloproteinases in the pathogenesis of genital endometriosis, further studies with large samples in various populations are needed.