Electrochemical Study of a Nano Vesicular Artificial Peroxidase on a Functional Nano Complex Modified Glassy Carbon Electrode

Abstract

A nano vesicular was constructed with the complex consisting of hemin, imidazole Gemini and sodium dodecyl sulfate (SDS) in 50 mM phosphate buffer solution (PBS). The novel nano structure acted as an efficient artificial peroxidase (AP). The AP was also immobilized on a carboxyl functionalized multi-walled carbon nano-tubes (MWCNTs-COOH) and gold nano-particles (AuNPs) nano-complex modified glassy carbon (GC) electrode, with a formal potential of -31 ± 2 mV vs. Ag/AgCl in 50 mM PBS, at a scan rate of 0.05 Vs -1. The heterogeneous electron transfer constant (ks) was evaluated to be 5.4 ± 0.1s-1. The modified electrode can be used to detect hydrogen peroxide in the range from 0.03 to 160 μM linearly, with a detection limit of 0.03 μM. The apparent Michaelis-Menten constant (Kmapp) of AP modified electrode was evaluated to be 0.034 ± 0.003mM.