Multiplex polymerase chain reaction method for the diagnosis of unspecified viral acute gastroenteritis

Abstract

Acute gastroenteritis (AG) can be caused by a wide range of viral pathogens. Detection of dominant pathogens (noro-, rota-, adeno-, enteroviruses) makes it possible to establish the etiology of 40-60% of AG cases. To increase the proportion of their etiological interpretation, it is advisable to conduct research on minor intestinal viruses. This article presents the method of multiplex polymerase chain reaction for the diagnosis of unspecified viral intestinal infection. It allows detecting the genetic material of astro-, sapo-, parecho-, boka-, picobirna- and aichi viruses in biological material (faeces). The approbation of the method made it possible to detect the presence of these pathogens in 4,89% of patients with AG, in whose biological material were no dominant intestinal viruses.