Mesenchymal stem cells from bone marrow of patients with drug-resistant tuberculosis for cellular therapy-Reference-Cited by-同舟云学术

Mesenchymal stem cells from bone marrow of patients with drug-resistant tuberculosis for cellular therapy

Published:2023-12-06 Issue:2 Volume: Page:40-45
ISSN:2074-2088
Container-title:Medical and Biological Problems of Life Activity
language:
Short-container-title:jour

Author:

Isaikina Y.,Solodovnikova V.,Frolova R.,Savich U.,Zhernasechanka H.,Skrahina A.

Abstract

In this study, we evaluated the proliferative capacity of mesenchymal stem cells (MSCs) from the bone marrow of patients with drug-resistant tuberculosis (DR-TB) and selected the optimal conditions for obtaining autologous MSCs transplant. The results of the expansion of MSCs from 42 patients with DR-TB in medium with 10% FBS demonstrated low proliferative potential for of these cells compared with MSCs from 47 donors. MSCs content in 1 ml of bone marrow of patients was also lower. A relationship was found between the amount of MSCs in the primary culture and in the finished product, and with a small number of cells in the primary culture, we used 5% platelet lysate instead of 10% FTS, which made it possible to obtain a high dose of MSCs in the transplant.

Publisher

Republican Scientific and Practical Center for Radiation Medicine and Human Ecology

Reference11 articles.

1. Secretome of Mesenchymal Stromal Cells Prevents Myofibroblasts Differentiation by Transferring Fibrosis-Associated microRNAs within Extracellular Vesicles // N. Basalova, [et al.] / Cells. – 2020. – Vol. 9, № 5. https://doi.org/10.3390/cells9051272.

2. Mora, A.L. Aging and lung injury repair: a role for bone marrow derived mesenchymal stem cells // A.L. Mora, M. Rojas. / J Cell Biochem. – 2008. – Vol. 105. – P. 641-647.

3. Allogeneic Human Mesenchymal Stem Cells in Patients With Idiopathic Pulmonary Fibrosis via Intravenous Delivery (AETHER): A Phase I Safety Clinical Trial / M.K. Glassberg [et al.] // Chest. – 2017. – Vol. 151. – P. 971-981.

4. Mesenchymal stem cells secrete immunologically active exosomes// Zhang B [et al.] / Stem Cells Dev. – 2014. – Vol23, №11. – P. 1233-1244.

5. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo // A.Bartholomew [et al.] / Exp. Hematol. – 2002. – Vol. 30. – R. 42-48.