Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation

Author:

Inoue Haruki1,Kunida Katsuyuki23,Matsuda Naoki3,Hoshino Daisuke34,Wada Takumi3,Imamura Hiromi5,Noji Hiroyuki6,Kuroda Shinya137

Affiliation:

1. Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo

2. Laboratory of Computational Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology

3. Department of Biological Sciences, Graduate School of Science, University of Tokyo

4. Department of Engineering Science, Graduate School of Informatics and Engineering, University of Electro-Communications

5. Department of Functional Biology, Graduate School of Biostudies, Kyoto University

6. Department of Applied Chemistry, Graduate School of Engineering, University of Tokyo

7. CREST, Japan Science and Technology Corporation

Publisher

Japan Society for Cell Biology

Subject

Cell Biology,Molecular Biology,Physiology,General Medicine

Reference36 articles.

1. Aoki, K. and Matsuda, M. 2009. Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors. Nat. Protoc., 4: 1623–1631.

2. Areta, J.L., Hawley, J.A., Ye, J.-M., Chan, M.H.S., and Coffey, V.G. 2014. Increasing leucine concentration stimulates mechanistic target of rapamycin signaling and cell growth in C2C12 skeletal muscle cells. Nutr. Res., 34: 1000–1007.

3. Baar, K. and Esser, K. 1999. Phosphorylation of p70(S6k) correlates with increased skeletal muscle mass following resistance exercise. Am. J. Physiol., 276: C120–7.

4. Bajcsy, P., Cardone, A., Chalfoun, J., Halter, M., Juba, D., Kociolek, M., Majurski, M., Peskin, A., Simon, C., Simon, M., Vandecreme, A., and Brady, M. 2015. Survey statistics of automated segmentations applied to optical imaging of mammalian cells. BMC Bioinformatics, 16: 330.

5. Ceelen, K.K., Oomens, C.W.J., Stekelenburg, A., Bader, D.L., and Baaijens, F.P.T. 2007. Changes in Intracellular Calcium during Compression of C2C12 Myotubes. Exp. Mech., 49: 25–33.

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