Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins

Author:

Kashiwagi Sayaka1,Fujioka Yoichiro1,Satoh Aya O.1,Yoshida Aiko1,Fujioka Mari1,Nepal Prabha1,Tsuzuki Atsushi1,Aoki Ozora1,Paudel Sarad1,Sasajima Hitoshi1,Ohba Yusuke1

Affiliation:

1. Department of Cell Physiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University

Publisher

Japan Society for Cell Biology

Subject

Cell Biology,Molecular Biology,Physiology,General Medicine

Reference58 articles.

1. Aliye, N., Fabbretti, A., Lupidi, G., Tsekoa, T., and Spurio, R. 2015. Engineering color variants of green fluorescent protein (GFP) for thermostability, pH-sensitivity, and improved folding kinetics. Appl. Microbiol. Biotechnol., 99: 1205–1216.

2. Archer, S.L. 2013. Mitochondrial dynamics—mitochondrial fission and fusion in human diseases. N. Engl. J. Med., 369: 2236–2251.

3. Aw, T.Y. and Jones, D.P. 1989. Heterogeneity of pH in the aqueous cytoplasm of renal proximal tubule cells. FASEB J., 3: 52–58.

4. Bajar, B.T., Wang, E.S., Lam, A.J., Kim, B.B., Jacobs, C.L., Howe, E.S., Davidson, M.W., Lin, M.Z., and Chu, J. 2016. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Sci. Rep., 6: 20889.

5. Berezhnov, A.V., Soutar, M.P.M., Fedotova, E.I., Frolova, M.S., Plun-Favreau, H., Zinchenko, V.P., and Abramov, A.Y. 2016. Intracellular pH modulates autophagy and mitophagy. J. Biol. Chem., 291: 8701–8708.

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