Abstract
Pityriasis Versicolor is a skin condition caused by the commensal yeast Malassezia. Little is known about the pathogenesis of why a commensal only causes symptoms in a subset of infected individuals. Understanding the susceptibility of the host to these commensal-associated diseases may be facilitated by knowledge of genetic polymorphism. The purpose was to investigate the relationship between Single Nucleotide Polymorphism in the IL10 and IFN genes of the host and susceptibility to Malassezia infection. There were 38 cases of Pityriasis Versicolor (PV) and 38 healthy controls in the sample. Blood samples were extracted for genomic DNA from all study participants. Amplification refractory mutations system- polymerase chain reaction (ARMS-PCR) with sequence-specific primers was used to genotype cytokines. In all patients and healthy controls, three SNPs (IL10-1082A/G; IL10-819/592C/T; IFN- +874A/T) in two cytokine loci were analyzed. In the PV group, we observed significant differences in allele or genotype distribution for the IL10-819/592C/T and IFN- +874A/T gene polymorphisms. In the present investigation, cytokine gene polymorphism revealed that the host was susceptible to Malassezia infection.
Subject
Industrial and Manufacturing Engineering,Environmental Engineering
Reference23 articles.
1. Ashbee HR, Evans EG. Immunology of Diseases Associated with Malassezia Species. Clin Microbiol Rev 2002;15:21–57.
2. Thomas DS, Ingham E, Bojar RA, Holland KT. In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species. FEMS Immunol Med Microbiol 2008;54:203–14.
3. Cafarchia C, Otranto D. Association between phospholipase production by Malassezia pachydermatis and skin lesions. J Clin Microbiol 2004;42:4868-9.
4. Choe YB, Jang SJ, Yim SM, Ahn KJ.The quantitative study on the distribution of Malasseziayeasts on the normal skin of the young adults. Korean J Med Mycol 2004;9:174-81.
5. Forke R, Jäger A, Knölker HJ. First total synthesis of clausine L and pityriazole, a metabolite of the human pathogenic yeast Malassezia furfur. Org Biomol Chem 2008;21:2481-3.