Growth, photosynthesis and pollen performance in saline water treated olive plants under high temperature

Abstract

Olive cultivation in hot arid areas is hindered by the scarcity of irrigation water. The exploitation of saline water has been proposed as a solution to partially cover plant water demands. This paper presents the effects of salinity [0, 60 and 120 mM sodium chloride (NaCl)] on physiological and reproductive functions of cultivars <em>Koroneiki</em> and <em>Amphissis</em> in a closed hydroponic system. Shoot growth was markedly reduced in high salinity dose in <em>Amphissis</em> (−81%) and <em>Koroneiki</em> (−75%). The photosynthetic rate was significantly reduced at 120 mM NaCl for both cultivars, as well as chlorophyll and carotenoids content (43% and 44%, respectively). The Na+ content in all plant parts increased in both salinity doses especially in <em>Amphissis</em> while K concentration decreased for both cultivars. Inflorescences in <em>Amphissis</em> were severely damaged due to salinity. Consequently, pollen sampling and <em>in vitro</em> germination study was only feasible for <em>Koroneiki</em>. Indeed, <em>Koroneiki</em> pollen germination was reduced at 60 mM NaCl (−42%) and at 120 mM NaCl (−88%). Pollen tube length was also reduced by 15% and 28% for the middle and high salinity dose, respectively. The results of the present study indicate that <em>Amphissis</em> is more sensitive in high salinity doses compared to <em>Koroneiki</em> and that reproductive functions are severely affected by salinity.