Author:
Thabet Marian,Abou-Zeid Mohamed A.,Safhi Fatmah A.,Alwutayd Khairiah M.,Khalifa Walaa
Abstract
Ultraviolet-C (UV-C) irradiation of grains activated the antioxidant system and wheat seedlings’ resistance to leaf rust and powdery mildew disease under greenhouse conditions. Two wheat cultivars (Gemmeiza-12 and Sids-1) with dry and germinated grains were treated with UV-C at three exposure times (5, 10, and 15 minutes). The results indicated that the percentages of disease severity and infection type for leaf rust and powdery mildew on wheat seedlings were significantly reduced when exposed to UVC at all exposure times compared to the untreated control. The most effective treatments for both cultivars were obtained in seedlings grown from germinated grains treated with UV-C for 10 minutes. Furthermore, UV-C irradiation treatments improved plant resistance to infection by activating certain defense genes, thereby increasing the production of resistance compounds that support defense mechanisms against pathogens. Our results demonstrated that UV-C for 10 minutes can induce resistance in wheat seedlings while also increasing total chlorophyll, total phenolic compounds, phenylalanine ammonia-lyase, and peroxidase activity. In addition, phenylalanine ammonia-lyase mRNA expression levels were significantly increased in seedlings growing from germinated grains treated with UV-C for 10 minutes, as compared to both infected and uninfected controls. These findings demonstrate the potential for additional UV-C radiation treatments to enhance disease resistance.
Subject
Agronomy and Crop Science
Reference79 articles.
1. Aarrouf J, Urban L, 2020. Flashes of UVC light: an innovative method for stimulating plant defences. PLoS One 15:e0235918.
2. Aboul Fotouh MM, Maha HM, Moawad FG, Tag El-Din MA, Srour HAM, 2019. Enhancement of resistance against Rhizoctonia solani by glycine betaine and UV-C radiation in green bean (Phaseolus vulgaris L.). Arab Univ. J. Agric. Sci. 27:1829-41.
3. Arab SA, El Shal MH, Eissa ST, Abou-Zeid MA, 2021. Assessment of some wheat genotypes for resistance to some diseases and their effect on yield and yield components. PCBMB. 22:73-8.
4. Araujo SS, Paparella S, Dondi D, Bentivoglio A, Carbonera D, Balestrazzi A, 2016. Physical methods for seed invigoration: Advents and challenges in seed technology. Front. Plant Sci. 7:646.
5. Atia AM, El-Khateeb EA, El-Maksoud RMA, Abou-Zeid MA, Salah A, Abdel-Hamid AME, 2021. Mining of leaf rust resistance genes content in Egyptian bread wheat collection. Plants 10:1378.