Abstract
The main objective of this work was the selection of mixed starters with a combination of Lactic Acid Bacteria (LAB) and yeast strains for traditional bread production in Morocco. For this, a total of 21 LAB strains and 36 yeast strains were isolated from different traditional sourdough. Dough fermentation were assessed by monitoring physicochemical parameters including, titratable acidity, decrease of pH and lactic acid, ethanol and CO2 production. A total of six yeasts and four LAB were selected for their technological performances. Morphological, physiological and biochemical identification performed using API identification kits confirmed that these strains belonged to Saccharomyes cerevisiae, Candida humilis and Saccharomyces exiguus species, and Lactobacillus plantarum and Lactobacillus casei species for yeasts and LAB respectively. The yeast S3-L2 and the LAB ODBL5 strains exhibited the best performances among the selected ones; S3-L2 yeast strain were able to produce ΔV=23mL of CO2 and showed the highest values of ethanol and biomass production (2.87 g/L and 1.25 10^9 UFC/mL, respectively). Whereas OD-BL5 LAB strains produced 13.9 g/L of lactate in dough. These findings lead to consider these two strains very good candidates for the formulation of an effective mixed starter for bread preparation. Subsequently, sensorial analysis results showed that bread prepared using mixed starter No. 24 composed of the two selected species exhibited better exterior appearance, golden and crispy crust, large volume and honeycomb crumb, compared to the control.
Subject
Biochemistry (medical),Plant Science,General Biochemistry, Genetics and Molecular Biology
Reference42 articles.
1. Kourkouta L, Koukourikos K, Iliadis C, et al. Bread and health. Pharm Pharmacol 2017;107:821–6.
2. FDA. Food Code. US Public Heal Serv 2017;0001:1–767.
3. Anjum FM, Pasha I, Ghafoor K, et al. Preparation of sourdough bread using a blend of bacterial culture and baker’s yeast. Nutr Food Sci 2008;38:146–53.
4. Urien C. Diversité des espèces de levures dans des levains naturels français produits à partir de farine issue de l’Agriculture Biologique: une étude pilote pour analyser les pratiques boulangères et les patterns des communautés microbiennes 2015. [Yeast species diversity in French natural sourdoughs produced from organically grown flour: a pilot study to analyse baking practices and microbial community patterns 2015] Ph.D. Thesis, [Gif-sur-Yvette]: Université Paris Sud-Paris, French.
5. Landis EA, Oliverio AM, McKenney EA et al. The diversity and function of sourdough starter microbiomes. Elife 2021;10:1–24.