Evaluation of antibacterial effects of Melia azedarach fruit extracts against some isolated pathogenic bacteria

Abstract

In ancient times plants have been a great source of medicine. Many of traditionally known plants have been extensively studied by advanced scientific techniques and reported for various medicinal properties such as anticancer, antinflammatory, antidiabetic, anthelmintic, antibacterial, antifungal, hepatoprotective, antioxidant, larvicidal. The aim of the present study was to evaluate the antibacterial effects of methanolic and aqueous extracts of <em>Melia azedarach</em> fruit against isolated strains of <em>Pseudomonas aeruginosa, E. coli, Staphylococcusaureus, Bacillussubtilis</em>, Proteus and Klibsiella using well and disc diffusion methods. Both extracts established significant antibacterial activity against tested bacteria. In well and disc methods, <em>Staphylococcus aureus, Pseudomonas aeruginosa</em>, Klibsiella and Proteus showed sensitivity at significant level to aqueous extract of Melia azedarach fruit. In comparison between two methods well and disc E. coli was significantly different at 50 mg/mL. and <em>Pseudomonas aeruginosa</em> at 100 and 200 mg/mL. <em>Staphylococcus aureus</em> and Proteus showed a significant difference in sensitivity to alcohol extract of M. azedarach fruit in well method. In disc method <em>Pseudomonas aeruginosa</em> and Proteus showed a significantly different sensitivity to alcohol extract of M. azedarach fruit. In comparison between well and disc method no significant difference was observed on alcohol extract of Melia azedarach. In comparison between alcohol and aqueous extract no significant difference was observed in both well and disc methods.