Comparison of the Etest and Sensititre methods for anaerobe susceptibility testing

Author:

Puechler KarinORCID,Scalzo KatiaORCID,Nicoletti Maira,Wieser Stefanie,Aschbacher Richard,Pagani Elisabetta

Abstract

Background and Aims: Antimicrobial susceptibility testing of anaerobic clinical isolates is of paramount importance for patient therapy and resistance monitoring. In our laboratory the MIC gradient Etest method and broth microdilution with Sensititre trays are used for susceptibility testing of anaerobes and the aim of this study was to compare the two methods on a panel of anaerobes routinely isolated from patients in the province of Bolzano, Italy. Materials and Methods: Totally, 74 non-repetitive Gram-positive and Gram-negative patient isolates were tested with Etest strips on Fastidious Anaerobe Agar (F.A.A.) and with Sensititre trays, according to manufacturer’s instructions. Interpretation of MICs was by EUCAST or CLSI criteria, resistance percentages were calculated and Categorical Agreement (CA) and Essential Agreement (EA) between the two methods were determined. Results: Of the 74 isolates, 68 (91.9%) grew on both systems and agreement for these was compared in the study. CA for all isolates was ≥90% for all tested antibiotics except moxifloxacin, whereas EA was generally lower. Resistance was generally low, except for clindamycin in all isolates and tigecycline in Gram-negatives. In our study Etest was a superior and more handy method. Conclusions: To conclude, we believe the Etest method is more suitable for routine diagnostic laboratory usage. Nevertheless, multicenter studies are required to evaluate the two methods for anaerobic susceptibility testing.

Publisher

PAGEPress Publications

Subject

General Medicine

Reference14 articles.

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3. Cherkaoui A, Fischer A, Azam N, et al. A comparison of SentititreTM Anaerobe MIC plate with ATB ANA® test for the routine susceptibility testing of common anaerobe pathogens. European Journal of Clin Microbiol & Infect Dis 2018;37:2279-84.

4. Clinical and Laboratory Standards Institute (CLSI). Methods for antimicrobial susceptibility testing of anaerobic bacteria: approved standard – eighth edition. M11-A8, Vol. 32 No. 5. Wayne, PA, USA: Clinical and Laboratory Standards Institute. 2012. Available from: https://clsi.org/media/1468/m11a8_sample.pdf

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