MORPHOLOGICAL CHARACTERIZATION AND GENETIC DIVERSITY USING THE SSR TECHNIQUE IN SOME APPLE GENOTYPES

Abstract

The scab is a widespread disease throughout the world causing large financial losses in apple production and needs to be controlled also by developing polygenic varieties with resistance to several races of the pathogen Venturia inaequalis by incorporation of two or more functionally different resistance genes. The studies for evidence of interest genes relating to the resistance of apple varieties to apple scab have been conducted using several types of molecular markers (SCAR, SSR, RAPD, ISSR etc.). In this experiment, using six SSR molecular markers (CH02b10, CH05e03, CH02d01, Hi07f01 and Hi07h02), was tracked the reveal of amplified fragments, corresponding to PCR products associated with resistance genes Rvi2, Rvi8, Rvi5 and Rvi11, but also the intraspecific diversity expressed at the molecular level of Romanian apple varieties, some of them having common genitors. The position of the amplified fragments on the agarose gel for the six SSR markers was located on similar values ranges to those published in various specialized papers, the size of the amplified fragments following to be evaluated by the sequencing step and published in a new paper as an addition to the results of this study.