Evaluation of Immunological Effects of Hochu-ekki-to (TJ-41) Prophylactic Administration in Mice

Abstract

We evaluated the immunological effects of a Kampo (Chinese) prescription Hochu-ekki-to (TJ-41) for 32 weeks and 1 week prophylactically in mice. The splenic natural killer cells (NK) of C57BL/6N mice prophylactically treated with TJ-41 for 32 weeks showed little enhanced cytotoxicity against NK-sensitive YAC-1 targets, but mice treated for 1 week showed significantly enhanced cytotoxicity. TJ-41 administration for 32 weeks increased the splenic NK cell population and CD4/CD8 significantly, but TJ-41 for 1 week was not affected. Further, there were no adverse effects of TJ-41 administration for 32 weeks. Whether or not that duration of administration can have the same beneficial effects on humans await further studies.