Enhancement of Nitric Oxide Production from Activated Macrophages by a Purified Form of Ginsenoside (Rg1)

Abstract

We studied the actions of purified ginsenosides Rg1 and Rb1 on nitric oxide production from macrophages and a macrophage cell line RAW264-7. Although neither Rg1 nor Rb1 induced nitric oxide from resting macrophages, Rg1 enhanced the production of nitric oxide from IFN-γ activated-macrophages or RAW cells. Rg1 also enhanced the production of nitric oxide from macrophages cocultured with nonadherent spleen cells stimulated by conA, LPS or anti-CD3. Rb1, however, did not significantly enhance nitric oxide production from stimulated macrophages or RAW cells. Rg1 enhanced the tumor cell killing by nitric oxide produced from IFN-γ-activated macrophages.