EXPLORE THE MECHANISM OF QUANTUM DOTS AS FLUORESCENT SENSOR TO DETECT HEAVY METAL IONS USING A SYNCHRONOUS FLUORESCENCE POLARIZATION TECHNIQUE

Abstract

By combining synchronous fluorescence (SF) spectroscopy and fluorescence polarization (FP) technology, a new spectral technique, termed synchronous fluorescence polarization (SFP) can be obtained. It inherits the advantages of both, such as high sensitivity, less sample demand, rapid detection, and so on. This concept has been proposed before without implementation details. In this work, a detailed implementation scheme of the technology is proposed, including the hardware requirements of the instrument, the measurement and calculation of the instrument correction factor G-factor, the spectral acquisition, the removal of inner filter effect (IFE), the removal of solvent background and the calculation and analysis of anisotropy. Furthermore, exploring the mechanism of quantum dots (QDs) as fluorescent sensor to detect heavy metal ions was taken as an example to introduce the application of the SFP method. Through detecting SF anisotropy, the mechanism of QDs probing [Formula: see text] ion was explained. If an appropriate fluorescent polarization enhancer is chosen, SFP can be used for quantitative detection. This work provides a specific implementation scheme of the SFP technology for the first time, which expands the application of fluorescence technology.